Discover how the Semrock optical filters business was started back in 2000.
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Multimodal nonlinear optical (NLO) imaging is a microscopy approach used in a variety of biomedical applications. It harnesses and integrates the unique capabilities of nonlinear processes such as multiphoton fluorescence, second- and third-harmonic generation (SHG and THG), and coherent Raman scattering (CRS) and combines them seamlessly into a single, unified microscopy platform.
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Optical edge filters are critical to the performance of Raman detection systems. This article describes two families of long‐wave‐pass (LWP) edge filters that Semrock has developed specifically for Stokes‐shift backscattered Raman signal detection systems. These filters are used in commercial Raman systems for polymer science, thin film analysis, geoscience, and the pharmaceutical industry
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Thin-film silicon (Si) photovoltaics are a combination of amorphous and microcrystalline Si; monitoring the proportions via Raman spectroscopy and high-performance optical filters allows the cells to be optimized during manufacture.
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Increasingly popular, total-internal-reflection-fluorescence (TIRF) microscopy systems prefer lasers for excitation because of their spectral purity and brightness. But the use of lasers can restrict other TIRF system components. Clearing these hurdles requires some careful considerations.
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594-nm laser excitation can enhance our ability to analyze cells by flow cytometry. This laser wavelength fills a significant excitation gap between the now common 561-nm line and the red laser sources. Adding this wavelength to the excitation repertoire enables excitation of virtually any visible fluorescent probe for cytometric analysis.
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Filter optimization is particularly important for applications where the FP signal and percentage of positive events are relatively low. In this article discover how the results substantially improved yield of GFP-expressing parasites, and requires decreased sorting time in comparison to standard methods.
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With carefully controlled absorption and scattering losses, high-reflectivity, low-loss dielectric mirrors enable demanding applications such as gravitational-wave detection and cavity ring-down spectroscopy.
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Optical filters are crucial in fluorescence experiments and instrumentation. Explore recent advances in filter technology offer a way to improve fluorescence techniques in this journal article.
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Optical filters with good performance in the mid-UV are crucial for enabling important new instruments based on applications including biochemical absorption, fluorescence and spectroscopy, as well as for realizing the full potential of industrial applications in areas including UV sterilization, and semiconductor and electronics manufacturing.
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Advances in filter technology enable improved multiphoton microscopy performance. Learn more about optical filters for multiphoton microscopy in this article for BioPhotonics.
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The development of reliable “hard” coatings for complex optical filters was an important trend in the optical coatings industry. Discover why areas such as biotechnology and analytical instrumentation shifted towards hard-coated filters to benefit not only from their performance, but also from their lasting reliability and durability.
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Based on ion-beam sputtering of hard oxide glass materials, the novel filters provide higher system brightness and sensitivity. Fluorescence microscopy is ideal for biological imaging because it reveals only objects of interest in an otherwise dark background, while permitting selective tagging. Learn more by exploring this journal article for BioPhotonics.
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High laser-induced damage threshold (LIDT) optical coatings help enable the highest-peak-power lasers in existence.
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The advent of lasers as fluorescence light sources imposes new constraints and demands on the optical filters required for optimal operation of these laser-based imaging systems.
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Efforts are under way to enhance the superresolution not only laterally but also axially. Rather than having to develop and use specialized fluorophores, many researchers have demonstrated the advantages of using standard fluorophores, expanding the applicability of super-resolution techniques.
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By using angle-tuned thin-film interference filters, optical engineers can capture nonstandard wavelengths during spectral imaging without compromising performance. Download this resource from OPN Optics & Photonics News now to learn more.
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With high-performance optical filters being incorporated into cutting-edge detection systems, Raman spectroscopy is finding new applications in the life sciences. Learn more in this journal article from BioPhotonics.
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When it comes to diagnostics, one of the most crucial – and often overlooked – components is the optical filter. But as manufacturing processes improve, optical filters of exceptional quality are being created, which not only deliver accurate results but also open up an entirely new avenue of application in wearable diagnostics.
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Active illumination microscopy is a simple and straightforward means by which to effectively control and manage the effects of photobleaching and phototoxicity in fluorescence microscopy. Through precise control of the illumination power, it is possible to eliminate image saturation while enhancing detection sensitivity at low signal levels. Because AIM requires only simple, straightforward electronics and only one detection channel, it can easily be employed as a simple “drop in” addition to any standard one- or two-photon laser scanning fluorescence microscope platform. Because of this, AIM should provide microscopists with another powerful method to enhance their imaging tool kit.
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