Stories & Features
Super-Resolution Microscopy Cubes
Jun 10, 2024
by
IDEX Health & Science
Super-resolution/TIRF Microscopy Cubes set the new standard for laser based microscopes. These cubes are optimizedfor mounting 1λ RWE 1mm thick super-resolution/TIRF laser dichroic beamsplitters. Maximize SNR and minimize artifacts in TIRF, Confocal, PALM, STORM, SIM, and other super-resolution techniques.
- Guaranteed 1λ P-V Relected Wavefront Error (RWE) from cube mounted 1 mm thick dichroic beamsplitters
- Maximize switching speed, minimize beam deviation, & minimize light scatter in emission
- Minimal reflected wavefront distortion for even large diameter illumination beams
- Available for popular laser lines & microscopy cubes (e.g. Nikon TE 2000, Olympus U-MF2 & U-FF, Zeiss FL Cube EC P&C)
Conventional microscopy cubes can significantly compromise the flatness of the dichroic beamsplitters thereby introducing aberrations.
But super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components for best instrument sensitivity. Our industry-leading 1λ RWE 1 mm thick laser dichroic beamsplitters minimize
focus shift and aberrations in the reflected beam compared to standard dichroic beamsplitters. However, in order to realize their full flatness potential, these dichroic beamsplitters need to be carefully mounted in microscopy cubes. Semrock has developed
proprietary methods of installing 1λ RWE super-resolution 1 mm thick dichroic beamsplitters in cubes and guarantee the flatness performance.
Offered as standard catalog products, cubes compatible with popular microscopes are available.
TIRF using 1 λ RWE Super-resolution Microscopy Cubes
Super-resolution Microscopy Cubes set the new standard for laser based microscopes. These cubes are optimized for mounting 1 λ RWE 1 mm thick super-resolution laser dichroic beamsplitters. Maximize SNR and minimize artifacts in TIRF, confocal, PALM, STORM, SIM, and other super-resolution techniques.
Conventional microscopy cubes can significantly compromise the flatness of the dichroic beamsplitters thereby introducing aberrations. But super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components for best instrument sensitivity. Our industry-leading 1λ RWE 1 mm thick laser dichroic beamsplitters minimize focus shift and aberrations in the reflected beam compared to standard dichroic beamsplitters. However, in order to realize their full flatness potential, these dichroic beamsplitters need to be carefully mounted in microscopy cubes. Semrock has developed proprietary methods of installing 1 λ RWE super-resolution 1 mm thick dichroic beamsplitters in cubes that guarantee the flatness performance. Offered as standard catalog products, cubes compatible with popular microscopes are available. 1 λ RWE 1 mm dichroics installed in standard microscopy cubes eliminate the need to re-align a 2 mm thick dichroic.
“The new TIRF & superresolution microscopy cubes from Semrock definitely provide more homogeneous and flat illumination in all excitation / emission bands, compared to other cubes I have seen so far.”
– Dr. Peker Milas, Ross Lab, University of Massachusetts Amherst
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| Xenopus laevis embryos transfected with GFP-cadherin and RFP-actin imaged with TIRF using Semrock’s Di03- R405/488/561/635-t1 super-resolution dichroic in a fullmultiband configuration. Image courtesy of Keck Imaging Center, University of Virginia. | Actin filaments imaged in TIRF using Semrock’s Di03- R405/488/532/635-t1 super-resolution dichroic in a full-multiband configuration. Image courtesy of Ross Lab, University of Massachusetts Amherst |
Single-band & Longpass
| Filter Set | Nikon TE 2000 | Olympus U-MF2 | Olympus U-FF | Zeiss FL Cube |
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Multiband
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