This "Pinkel" multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 532 nm laser source for TRITC, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/488/532/635-4X-A
Di03-R405/488/532/635-t1-25x36FL-007029
Each
• Laser Dichroic Beamsplitter with edges at 418.8 nm, 497.6 nm, 541.9 nm & 659.1 nm
• Rabs > 94% 370 – 410 nm, 473 – 491 nm, 530.5 – 533.5 nm & 632.8 – 647.1 nm • Ravg > 90% 350 – 370 nm • Tavg > 93% 426 – 462 nm, 502.5 – 518.5 nm, 550 – 613 nm & 663 – 1200 nm • Flatness / RWE: Super-resolution / TIRF |
FL-007029 |
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FF01-390/40-25FL-004666
Each
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FL-004666 |
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FF01-446/510/581/703-25FL-004412
Each
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FL-004412 |
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FF01-532/3-25FL-004387
Each
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FL-004387 |
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FF01-640/14-25FL-004697
Each
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FL-004697 |
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FF02-482/18-25FL-004712
Each
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FL-004712 |
S-000429 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
S-001718 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
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Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
A-327