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BrightLine® Sedat 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 561, & 635 nm laser sources

Semrock Part Number: LF405/488/561/635-4X4M-B-000

IDEX Part Number: S-003056  /

Semrock

This "Sedat" multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 559, 561.4 or 568.2 nm laser source for mCherry, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/488/561/635-4X4M-A

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 3,900.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
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Set Components

Di03-R405/488/561/635-t1-25x36

FL-007139

Each
• Laser Dichroic Beamsplitter with edges at 418.5 nm, 498.3 nm, 576.5 nm & 658.1 nm
• Rabs > 94% 370 – 410 nm, 473 – 491 nm, 559 – 568.2 nm & 632.8 – 647.1 nm
• Ravg> 90% 350 – 370 nm
• Tavg > 93% 426 – 462 nm, 502.5 – 544.5 nm, 582 – 617.5 nm & 663 – 1200 nm
• Flatness / RWE: Super-resolution / TIRF

FL-007139

FF01-390/40-25

FL-004666

Each
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

FL-004666

FF01-445/20-25

FL-004531

Each
  • Tavg > 93% 435 – 455 nm
  • Center Wavelength of 445 nm and GMBW of 20 nm
  • FWHM of 25 nm

FL-004531

FF01-525/30-25

FL-004450

Each
  • Tavg > 90% 510 – 540 nm
  • Center Wavelength of 525 nm and GMBW of 30 nm
  • FWHM of 35.8 nm

FL-004450

FF01-563/9-25

FL-004686

Each
  • Tavg > 93% 559 – 568 nm
  • Center Wavelength of 563.5 nm and GMBW of 9 nm
  • FWHM of 14.5 nm

FL-004686

FF01-605/15-25

FL-004393

Each
  • Tavg > 90% 597.5 – 612.5 nm
  • Center Wavelength of 605 nm and GMBW of 15 nm
  • FWHM of 22 nm

FL-004393

FF01-640/14-25

FL-004697

Each
  • Tavg > 93% 633 – 647 nm
  • Center Wavelength of 640 nm and GMBW of 14 nm
  • FWHM of 20.2 nm

FL-004697

FF01-676/29-25

FL-004567

Each
  • Tavg > 90% 661 – 690 nm
  • Center Wavelength of 675.5 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FL-004567

FF02-482/18-25

FL-004712

Each
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

FL-004712

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Center Wavelength 1 375 nm
Center Wavelength 4 635 nm, 640 nm
Edge Wavelength 1 405 nm, 638 nm
Laser Wavelength 1 (high) 491 nm, 568.2 nm
Laser Wavelength 1 (low) 473 nm, 561.4 nm, 632.8 nm, 647.1 nm
Laser Wavelength 2 488 nm
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

LF405/488/561/635-4X4M-B-NTE

S-001149 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
2-dodecylresorufin-lipid 582 595
4-methylumbelliferone 357 441
5-carboxy-2,7-dichlorofluorescein 500 525
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
7-hydroxy-4-methylcoumarin 360 442
Acridine Orange (+DNA) 502 526
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 568 579 603
Alexa Fluor® 633 631 647
Alexa Fluor® 635 633 648
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
Alexa Fluor® 680 679 702
AMCA (Aminomethylcoumarin) 350 448
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 488 500 525
ATTO 495 495 527
ATTO 565 563 592
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
ATTO 680 680 700
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BD Horizon V450 406 449
BFP (EBFP) 380 440
BOBO™-3 570 605
BODIPY 630/650-X 625 641
BODIPY 650/665-X 646 664
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcofluor White 351 444
Carboxynaphthofluorescein 599 674
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy5.5™ 672 690
Cy5™ 649 666
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DAPI 359 461
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DiFMU-pH 9.0 358 450
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-505-Phalloidin 503 530
DY-590 582 599
DY-630 634 656
DY-675 675 699
DyLight 405 398 420
DyLight 633 620 649
DyLight 649 651 673
DyLight 680 676 705
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
ER-Tracker™ Blue-White DPX 372 557
Ethidium bromide 518 603
Ethidium homodimer 527 617
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 405 403 428
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 647 649 673
HiLyte Fluor™ 680 688 702
Hoechst 33258 352 455
Hypericin 600 603
IRDye 680 NHS Ester 683 710
IRDye 700Phosphoramidite 691 699
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LOLO-1 568 580
LysoSensor Blue 374 424
LysoSensor Green 448 502
LysoTracker Blue 373 422
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mApple 566 594
Marina Blue 363 461
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Deep Red 641 662
MitoTracker™ Green 490 512
MitoTracker™ Red 578 598
mKate2 588 633
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
Pacific Blue™ 404 455
pHrodo™, succinimidyl ester 560 587
PicoGreen® 502 522
PKH67 488 500
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 525 Nanocrystals 300 525
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Quasar® 705 688 706
ratiometric pHluorin pH5 500 509
ReAsH-CCPGCC 592 606
Red 580 580 603
Red 650 649 665
Resorufin 571 584
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
Rhodol Green 497 524
sgBFP™ 387 451
sgGFP™ (super glow GFP) 472 506
Sirius 355 424
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
Sodium Green 507 532
SpectrumBlue 405 449
SpectrumFRed (Far Red) 650 673
SpectrumGreen 497 538
SpectrumOrange 554 587
Sulforhodamine 101-EtOH 578 593
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 17 619 638
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
SYTOX Red-DNA 640 658
TagBFP 402 457
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
TO-PRO-1 515 531
TO-PRO-3 642 657
TOTO-1 514 531
TOTO-3 642 661
TRITC (Tetramethylrhodamine) 552 578
TurboGFP 482 503
TurboRFP 553 573
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Violet 369 437
wtGFP (wild type GFP, non-UV excitation) 474 509
X-Rhod-1 Indicator 580 601
YO-PRO-1 491 506
YOYO-1 491 508

Specifications

LF405/488/561/635-4X4M-B-NTE

S-001149 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

Compare Compare
More Info
Compare Compare
Hide Show
{{compareProduct.AlternativeText}}
{{compareProduct.AlternativeText}}

/ 

1 of 1
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

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