Stories & Features
NEW WEBINAR: Spectral Modeling of Fluorescence Crosstalk & Filter Edge Position Jitter
Dec 5, 2024
by
IDEX Health & Science
Did you miss our live webinar?
Watch the replay below!
The session discusses the selection of optical filters in a fluorescence microscope system for biological applications, under real-world conditions of overlapping fluorescent probe spectra and spectral edge position jitter in bandpass and dichroic filters. Overlapping spectra result in crosstalk/unmixing issues that complicate both the interpretation of data obtained from a typical fluorescence detection system and the choice of optimal optical filters. Similarly, spectral edge variation in optical filters, due to imperfections in production processes, can result in device-to-device variability in detected signal levels.
Simulation studies can be useful to understand the origins and extent of these issues and can assist in the optimal choices for filters and components such as excitation light sources. This session will provide guidance on how to perform these simulations with SearchLight, a free tool available from IDEX Health & Science | Semrock to simulate an epi-illumination microscope, that comes with a large database of fluorophores, optical filters, light sources, and cameras. The presentation is ideal for optical system engineers in fluorescence device development, university researchers using and developing fluorescence microscopes and systems, and consultants to that segment of the optical device industry.
SearchLight Benefits:
- Evaluate and optimize your system configurations
- Save, Share, & Export your results for better collaboration
- Take advantage of the regularly updated databases
- Save time in the lab and make every photon count with SearchLight