This Full-multiband triple-band set is designed for imaging CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in triple-band performance, and has been optimized for popular LED-based light engines. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.
Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.
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FF01-475/543/702-25
FL-004065 / 0
- Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
- Center Wavelengths of 475 nm, 543 nm & 701.5 nm and GMBWs of 22 nm, 22 nm & 197 nm
FF01-438/509/578-25
FL-004375 / 0
- Tavg > 93% 426 – 450 nm, Tavg > 93% 497.6 – 519.5 nm & Tavg > 93% 567.4 – 588.6 nm
- Center Wavelengths of 438 nm, 509 nm & 578 nm and GMBWs of 24 nm, 21.9 nm & 21.2 nm
FF459/526/596-Di01-25x36
FL-007091 / 0
- Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
- Ravg > 95% 350 – 450 nm, Ravg > 95% 426 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
- Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| 6-JOE | 520 | 548 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| Alexa Fluor® 514 | 518 | 543 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| Alexa Fluor® 660 | 663 | 691 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 425 | 436 | 484 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| ATTO 520 | 516 | 538 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| ATTO 590 | 594 | 624 |
|
|
| ATTO 594 | 601 | 624 |
|
|
| ATTO 610 | 614 | 634 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY R6G | 528 | 547 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| DDAO | 648 | 657 |
|
|
| Dronpa | 502 | 516 |
|
|
| DY-415 | 418 | 470 |
|
|
| DY-505-Phalloidin | 503 | 530 |
|
|
| DY-590 | 582 | 599 |
|
|
| DY-630 | 634 | 656 |
|
|
| DyLight 488 | 493 | 518 |
|
|
| DyLight 594 | 593 | 618 |
|
|
| ECFP | 435 | 475 |
|
|
| Eosin | 525 | 546 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| FM 4-64 | 505 | 725 |
|
|
| Gold 525 | 521 | 551 |
|
|
| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
|
| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| JO-JO-1, JO-PRO-1 | 530 | 544 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LDS 751 | 560 | 711 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| Magnesium Green | 507 | 531 |
|
|
| mApple | 566 | 594 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| mCherry | 587 | 610 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mPlum | 589 | 649 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTFP1 | 462 | 492 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
|
| PerCP | 490 | 677 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| POPO-1 | 433 | 457 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SpectrumRed | 587 | 615 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYTO 11 | 506 | 525 |
|
|
| SYTO 17 | 619 | 638 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| TagCFP | 458 | 480 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red-X® | 595 | 613 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| TurboYFP | 525 | 538 |
|
|
| Venus | 516 | 528 |
|
|
| VIC | 530 | 550 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YFP (yellow GFP) | 513 | 530 |
|
|
| YO-PRO-3 | 613 | 629 |
|
|
| YOYO-3 | 611 | 631 |
|
|
| ZsYellow1 | 529 | 539 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-475/543/702-25
FL-004065 / 0
- Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
- Center Wavelengths of 475 nm, 543 nm & 701.5 nm and GMBWs of 22 nm, 22 nm & 197 nm
FF01-438/509/578-25
FL-004375 / 0
- Tavg > 93% 426 – 450 nm, Tavg > 93% 497.6 – 519.5 nm & Tavg > 93% 567.4 – 588.6 nm
- Center Wavelengths of 438 nm, 509 nm & 578 nm and GMBWs of 24 nm, 21.9 nm & 21.2 nm
FF459/526/596-Di01-25x36
FL-007091 / 0
- Dichroic Beamsplitter with edges at 459 nm, 526 nm & 596 nm
- Ravg > 95% 350 – 450 nm, Ravg > 95% 426 – 450 nm, Ravg > 95% 497.6 – 519.5 nm & Ravg > 95% 567.4 – 588.6 nm
- Tavg > 93% 464 – 486 nm, Tavg > 93% 532 – 554 nm & Tavg > 93% 603 – 800 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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